TLC lab: this lab's week was really hard. can any one help me out with these questions?

Posted on 2015-10-13 06:50:00 in Chemistry by ARSHIA

1. What would be the result of failing to mark the solvent front after developing a TLC plate? Would this affect your ability to calculate component Rf values? Why or why not?

2. What would happen if your origin line was oriented so low on the TLC plate that when it was lowered into the TLC chamber it was positioned below the level of the developing solvent (eluent)?

3. Assuming that the separation of a binary mixture in ideal circumstances (concentrated spotting) would be
relatively small (less than 0.5 cm), what would be the result of applying too large a spot of this mixture to the
TLC plate? How would it affect your ability to distinguish one component from the other on the plate? How
would it affect your Rf values? Draw a sample TLC plate to help illustrate your answer.

4. Unknown compound Q is spotted on a TLC plate that is then developed in cyclohexane. The solvent front is measured at 5.2 cm and the distance traveled by compound Q is measured at 3.4 cm. A sample of
acetaminophen is spotted on a TLC plate that is then developed in cyclohexane. The solvent front is
measured at 4.15 cm and the distance traveled by acetaminophen is measured at 2.70 cm. What can be
determined about the identity of compound Q in light of this data?

5. Describe how TLC could be used to monitor a reaction’s progress (product & byproduct formation) in a
research/teaching laboratory.

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