Biology Questions?

Posted on 2015-10-19 06:02:00 in Biology by EIAN

Question #1: Which is a TRUE statement regarding PCR and Sanger Cycle Sequencing?

- Both use dNTPs, and both use both forward and reverse primers
- Both use dNTPs; PCR uses forward and reverse primers, and Sanger Cycle Sequencing uses only one primer
- Sanger Cycle Sequencing uses ddNTPs instead of dNTPs; PCR uses forward and reverse primers, and Sanger Cycle Sequencing uses only one primer
- Sanger Cycle Sequencing uses both ddNTPs and dNTPs. Both PCR and Sanger Cycle Sequencing use only one primer

Question #3: Which of the following is NOT an advantage of using GelRed in the gels?

- It is cheaper than ethidium bromide or SYBR green
- It enables you to see the DNA after electrophoresis
- It is not toxic, so one does not have to wear gloves, as we do with ethidium bromide or SYBR green
- It can be stored at room temperature

Question #4: Which is FALSE regarding ddNTPs used in Sanger Cycle Sequencing?

- They create various fragment lengths of DNA
- They are labeled in order to distinguish between each nucleotide (ddATP, ddCTP, ddGTP, and ddTTP)
- dNTPs are added in the 5’ to 3’ direction, whereas ddNTPs are added in the 3’ to 5’ direction
- They attach to dNTPs, but dNTPs cannot attach to them

---

Answer Comment

No Answers Posted Yet.