Purification of proteins is a difficult task since proteins are very fragile and temperature sensitive molecules. First, a search is to be made to select a suitable source that will yield the desired protein in measurable quantity. The source is a fresh tissue which is subject to the action of a waring Blender for grinding to obtain a homogenate that rich in protein as well as contaminating material. The homogenate is filtered and freed from unwanted material by treatment with suitable solvents. Since proteins get denatured easily, care must be taken to maintain ideal pH and temperature. Generally purification is carried out in the presence of suitable buffers and at temperatures close to the freezing point. Some of the techniques used for purification of proteins will be discussed here.


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